Palestra no Centro de Ecologia Funcional Univ. Coimbra. - 7 de Maio
01-05-2010 22:20
Dentro das palestras organizadas pelo CFE, o João Loureiro (jloureiro@bot.uc.pt) e a Sílvia Castro (scastro@bot.uc.pt) vão realizar uma interessante apresentação sobre:
Applications of flow cytometry in plant population biology and ecology
Flow cytometry (FCM) is a high-throughput technique that simultaneously measures and analyses multiple parameters of individual particles. Over the last decade, the applications and use of FCM in plant ecology have increased dramatically. The unsurpassed speed and reliability on estimating differences in nuclear DNA content by FCM allowed large-scale surveys at the landscape, population, individual and tissue levels, with the majority of the studies being focused in spatial distribution and evolutionary significance of genome duplication (polyploidy). Also, representative sampling opened the possibility to gain novel insights into the extent of intra- and inter-population ploidy variation, niche differentiation, and ecological preferences of particular cytotypes. In combination with molecular and phenotypic approaches, FCM promises qualitative advances in our understanding of genome multiplication and the population biology of vascular plants. In the present talk we will expose the functioning of this technique and explore its importance in the areas of plant population biology and ecology, namely by presenting several case studies (e.g., cytotype distribution in Aster amellus agg. and Gymnadenia conopsea agg., invasive species Oxalis pes-caprae) that we have been exploring in the past years.
Próxima sexta feira dia 7 de Maio as 11 da manhã no anfiteatro do ex-Departamento de Botânica.